RRRRRRRR RRRRRRRR RR RR eeeee RR RR ee ee aaa RRRRRRRR eeeee a a dme.txt RRRRRRRR ee aaaa RR RR eeeee RR RR RR RR RR RRR OpenLAB CDS ChemStation Edition Revision - C.01.05 – February 2013 Please read this information carefully. It supersedes the printed material you received with your software. ________________________________________________________________________________ CONTENTS ________________________________________________________________________________ 1 Introduction 2 Configurations 1. 1100/1200 Instrument Configurations (no G1315C&D / G1365C&D Detectors) 1.1 1100/1200 Instrument Configurations: Additions using G1315C&D/G1365C&D Detectors 1.2 G7100 CE Instrument Configurations 1.3 Instruments connected to an AIC 2. Printing Hints 3. Method resolution will occur if there has been a change in the hardware configuration of the instrument 3.1 Method Resolution for GC instruments 3.2 Method Resolution for LC instruments 3.3 New concept for the LC sampler thermostats 4. Mixed Configuration with ChemStation UV-Visible 5. GC ChemStation demonstration Methods and Data files 6. Winexec fails 7. 6890 NP Detector 8. Injector configuration changes from the 6890 Key Pad 9. 5890 Series II and 4890D support statement 10. AIA/ANDI problems: import of files 11. AIA/ANDI problems: Printing Reports to Files 12. Using Batch Review 13. Report: XLS file output 14. Barcode Reader use with methods 15. The 7673A/B/C family of ALS products are no longer supported 16. 35900E A/D Converter 17. Retention Time Locking Notes 18. LC/MS ChemStation Instrument Models supportability matrix 19. Power Management and Screen Savers and the ChemStation 20. Support Statement for Microsoft Windows Vista 3 Known Issues 4 Obtaining Support _______________________________________________________________________ _______________________________________________________________________ 1 Introduction Welcome to the new OpenLAB CDS ChemStation Edition C.01.05 for GC, LC, LC/MS, CE, CE/MS, and A/D Systems. OpenLAB CDS ChemStation Edition brings a number of improvements in functionality, stability, and productivity to customers in many industries. For details regarding features added in this and previous revisions of OpenLAB CDS ChemStation Edition see the CS_ReleaseNotes.pdf located on disk 2. _________________________________________________________________________ 2 Configuration 1. 1100/1200 Instrument Configurations (without G1315C&D / G1365C&D Detector) ----------------------------------------------------------------- 1100/1200 instruments require LAN communication. The configuration below is based on the detector as carrier of the LAN communication card. 1100/1200 chromatography instrument configurations on a single workstation, without diode array detectors (DADs), are limited to a total of around 18 individual modules, configured as up to 4 instruments with a limit of 4 detectors per instrument. The maximum number of 18 modules can consist of approx. 12 modules like pumps, detectors, injectors, etc. and approx. 6 can-slave modules like valves, UIB interfaces. The maximum number depends on the exact module configuration and LAN connection point. If detectors of the same type are used in one stack, 2 detectors of the same types are supported, in case of the VWD up to 3. If DADs are included in the configuration there is a limit of 2 DADs and around 8 modules that may be configured as two separate instruments. In case of mixed DAD/MWD configurations ("C/D" plus "A/B") a number of medium issues were encountered during test that are related to this mix. Standard operation is not a problem, but * the Isoabsorbance and 3D Plots are not working correctly * in the method printout both DADs have the same header * only one DAD can be selected in Diagnosis view if another DAD is present * not all signals are printed in the report in these systems * in online plot it is difficult to differentiate between two detectors Depending on the number of modules attached to the system and the differences in the applications in use (e.g. data rate), we recommend to contact your Agilent Representative to identify the supported configuration for very complex systems. When an instrument configuration includes the LC/MS, there is a limit of 1 LC/MS and 1 DAD on the instrument and no additional instruments are supported on the workstation A Purification System configuration with 1100/1200 Fraction Collector(s) is limited to only one instrument. While acquiring data in three and four instrument configurations, interactive data reprocessing is not recommended. 1100/1200 modules may be used in mixed configurations with Agilent gas chromatographs (GC). The configuration limits for these instrument combinations are: 1100/1200 LC mixed module combinations: The 35900E Dual Channel Interface counts as the equivalent of 1 1100/1200 module. 1100/1200 and GC combinations: 1 - 1100/1200 instrument, without DAD, with up to 3 - Agilent GCs 2 - 1100/1200 instruments, with DAD, with up to 2 - Agilent GCs. 1.1. 1100/1200 Instrument Configurations: Additions using G1315C&D / G1365C&D Detectors ----------------------------------------------------------------------------- The G1315C&D/G1365C&D require LAN communication and the detectors have been equipped with on-board LAN communication. The detectors must be the communication entry point to the PC if used in an 1100/1200 stack. Using an 1100/1200 chromatography instrument configurations including the G1315C&D detector (DAD), the system is limited to a total of approximately 18 individual modules configured as up to 2 instruments with a limit of two G1315C/D DAD detectors per PC. The maximum number of 18 modules can consist of approx. 12 modules like pumps, detectors, injectors, etc. and approx. 6 can-slave modules like valves, UIB interfaces. The maximum number depends on the exact module configuration. The G1315C&D/G1365C&D detector (DAD/MWD) can be used together with G1315A/G1365A as well as G1315B/G1365B. In case of mixed DAD system, the DAD control in the Method and Run view has to be handled using the menu items, not the GUI. When running Diagnosis, only one detector can be configured in order to run the test. In case of a mixed detector system, the configuration needs to be changed to only one detector prior running the Detector Diagnosis tests. 1.2. G7100 CE Instrument Configurations ---------------------------------------------------- When an instrument configuration includes the CE/MS, there is a limit of 1 CE/MS including the DAD on the instrument. No additional instruments are supported on the CE/MS or CE ChemStation. 1.3. Instruments connected to an AIC (Agilent Instrument Controller) ------------------------------------------------------------------- Only instruments with a LAN connection can be controlled by an AIC. This excludes the ELSD detectors using RS232. In addition no MS detectors can be configured on an AIC. One AIC can have up to 5 DADs or 10 2-D instruments such as a GC or a LC with no DAD. A 3D/DAD instrument is equivalent to two 2D instruments, therefore, an AIC can control e.g. 3 LCs with DAD and up to 4 Agilent GCs. 2. Printing Hints ------------------- Please make sure that a default printer is configured in Windows. This is done via the Control Panel application. If no default printer is configured the following problems have been reported: - Printing of a report preview will fail. - the 'Copy To Clipboard' menu will have an error - the 'Custom Report View' will have problems with new templates PDF XChange printer During the installation of the ChemStation C.01.05 a PDF XChange 4 printer driver is installed (revision 4.0.194.0). Limitations - The maximum number of pages for one print job is 1500, for example sequence summary report is one print job. - When printing "Multi Page" chromatograms (see: Specify Reports) the maximum number of pages which can be printed properly depends on the resolution of the printer. 300 dpi allows 10 pages, 600 dpi allows up to 5 pages per chromatogram. Changing Printer Settings Before changing printer settings close the "Report"-window. When a report is generated on screen (in a window titled "Report") this is as if the report was already printed. This means changing the printer settings while the "Report"-window is already open does not influence the already generated report. In other words: hitting the print button in the "Report"-window does not generate the report anew! It just 'copies' the report to the printer. To allow checking for which printer the report was generated the "Report"-window gives in its lower left corner the name of the printer, the paper format and the orientation of the currently visible report. If this does not match the current printer configuration, then you will get a mis-formatted printer output. Report Viewer Beginning in C.01.03, a report viewer has been introduced that allows viewing existing PDF or Txt reports from disk. The previous option -opening the file from Windows explorer- had disadvantages during ECM upload. I also created problems during reprocessing, when report files were open. Reports can be viewed by a right click on the corresponding data file in the Navigation Table and selecting View Report File(s) from the context menu. 3. Method resolution will occur if there has been a change in the hardware configuration of the instrument -------------------------------------------------------------------------- 3.1 Method Resolution for GC instruments Method resolution will occur if there has been a change in the hardware configuration of the instrument, such as adding a new inlet or detector. After a change in the hardware configuration, the method must be updated before use. 3.2 Method Resolution for LC instruments RC.Net drivers only: In case of solvent or column switching valves, method resolution is triggered by spelling differences of solvent names or column serial numbers as the valve position is directly controlled by the solvent name or serial number stored in the method. After a change in the hardware configuration, the method must be re-saved. 3.3 New concept for the LC sampler thermostats In earlier revisions the temperature control of the coolers for LC samplers and fraction collectors has not been a method setting. Beginning in revision C.01.03, a new concept was introduced that allows choosing between system setting or method setting. The setting can be selected in the configuration screen of the sampler. It is not available for thermostatted fraction collectors. - Temperature is not controlled by the method. This is the equivalent of the old setting - Temperature is controlled by the method and the method parameter can be either uncontrolled or a set value. Users need to be aware that a method that sets a temperature value for the next injection will affect all other positions in the tray. This can lead to vials that are warmer than desired decomposition) or colder than desired (precipitation). 4. Mixed Configuration with ChemStation UV-Visible ------------------------------------------------------- The ChemStation for GC, LC, CE, A/D, CE/MS, and LC/MS, revision C.01.0x is not supported for co-execution or co-installation with the ChemStation for UV/Visible. 5. GC ChemStation demonstration Methods and Data files ------------------------------------------------------- When you install a GC instrument, several demonstration methods and data files are placed in the instruments subdirectory. The methods are placed in the methods subdirectory (for example; Chem32\1\METHODS). The data files are placed in a subdirectory called 'DEMO' under the data subdirectory (for example; Chem32\1\DATA\DEMO). Three of the methods have associated data files. The table below explains the methods and datafiles. METHOD DATA FILE PURPOSE --------- --------- ------- ESTD_EX.M ESTD1.D This is a calibrated external standard method ESTD3.D with three levels of calibration for two peaks. ESTD5.D The three ESTD datafiles are the calibration files. They are 5890 data files. ISTD_EX.M ISTD1.D This is a calibrated internal standard method ISTD3.D with three levels of calibration for two peaks. ISTD5.D The three ISTD datafiles are the calibration files. They are 5890 Series data files. 100FID.M 100FID5.D This method can be used to display the three 100FID20.D 100FID files. These are examples of the Ultra 100FID50.D column test mix run on a 10m X 100uM id X 0.17uM film HP-5 column in an 6890 Series GC. They were collected at 5, 20, and 50 Hz data rates, respectively. 6. Winexec fails ---------------- The GP-IB communication libraries were not installed if the error message "hsictsk, winexec failed; err=2" is obtained when starting the ChemStation. Solution: Install the GP-IB communication libraries, configure the GP-IB device and restart the ChemStation. 7. 6890 NP Detector -------------------- NP detector bead settings. It is important, before changing your NP bead, to set the voltage to zero (do not turn it off, set it to zero) from the 6890's front panel, then save the ChemStation method. This way, when you power the 6890 and ChemStation back on after changing the bead, a safe voltage will be applied to the new bead. From there, follow the factory's new-bead-conditioning procedure. 8. Injector configuration changes from the 6890 Key Pad -------------------------------------------------------- When changing injector configuration from the 6890 key in a dual injector configuration, the changes may not immediately be reflected in the ChemStation. Workaround: Close and restart the ChemStation. Any injector configuration changes will now be reflected in the ChemStation. 9. 5890 Series II and 4890D support statement ----------------------------------------------- The 5890 Series II GC and 4890D are out of support and have not been tested with software revision C.01.XX. It is recommended to use the last revision of the GC ChemStation that supported the 5890II and 4890D GC which is B.04.01. 10. AIA/ANDI problems: Import of files --------------------------------------- There is no default AIA events table supplied with the method. The auto integrate macro will supply the correct integration parameters. The way to get a properly shaped and integrated AIA signal into the ChemStation is: 1. Load the AIA file 2. Switch to the integration events user interface 3. Use the auto integrate button (parameters will load into visible events table) 4. Review parameters 5. Exit and save the events table After these steps there exists a default table for this type of AIA file, that will be used on subsequently imported AIA data. If the AIA data files come from a different source, the scale might be different again, and step 2 - 5 might have to be repeated. If the AIA file is exported after going through steps 1 - 5, it will be stored in the correct format. 11. AIA/ANDI problems: Printing Reports to Files ------------------------------------------------- Problem: When printing a report to file for an imported AIA file (non-Agilent), the report file cannot be created. Solution: This happens since non-Agilent AIA files are not directories. Export the AIA file from the ChemStation. This creates a directory containing the AIA file. For example: myfile.aia\signal01.cdf. Now report to file is able to generate files in the myfile.aia directory. Problem: Import of AIA files originating from a LAS system and converted to the AIA format using a utility supplied by Integ Germany may fail with a General Protection Fault. Solution: Since the company Integ Germany no longer exists, no fix is available for this issue. 12. Using Batch Review ---------------------- Batch review can be used to review results runs. There are a few points to watch out for: 1. The recalibration methodology in Batch Review differs from the recalibration in the original sequence. Pressing the "update calibration" button on the batch review toolbar causes the system to recalibrate with all calibration runs in the batch, creating a recalibrated calibration table. Pressing the "Start" button on the Batch Review toolbar will then recalculate amounts for each calibrated compound. The amounts for all samples will be calculated from the recalibrated calibration table. 2. Clicking a line on the Batch Review sample table causes the associated data file to be loaded. Some large data files may take a significant amount of time to load. This is no different than using Load Signals to load the same data file. 3. The Batch Review report gives results for each compound for each sample. Because some calibration tables have large numbers of compounds this may lead to quite large Batch Review reports. 4. Using the ChemStation with the ECM connection installed, the Batch view is by default disabled. It can be enabled by an entry in the [PCS] section of the ChemStaion.ini file: [PCS] _BatchReview=1 The ChemStation.ini file is located in the C:\Windows directory. 5. Batch Review allows saving manual integration events per run. It is also possible to save manual events with the data file outside of Batch Review. To avoid conflicts with two sets of manual integrations, any manual events that have been stored with the data file will not be applied in Batch Review. 13. Report: XLS file output (Classic reporting only) ---------------------------------------------------- Date/Time fields - Some fields (such as "DAMethModTime" and "AcqMethModTime" on "Sheet1" are date/time values. The value is the number of seconds elapsed since midnight (00:00:00), January 1, 1970, Universal Coordinated Time. Excel date/time values are the number of days from 1/1/1900 with the fraction being the time. To convert these values: 1. Divide by the number of seconds in a day (60*60*24 = 86400) 2. Add the number of days from 1/1/1900 to 1/1/1970 (25569 when corrected for leap-year problems in Excel, and for the fact that 1/1/1900 is "1" in Excel.) The new value will be the date/time in Universal Coordinated Time. Conversion to local time is left to the user of the spreadsheet. Precision - All values given are in the full internal representation of the value. In other words, no "truncation" has been done by the ChemStation. Follow standard guidelines regarding the number of significant digits when designing a report based on values from the spreadsheet. Help - Some useful help topics include "Integrator Results Table" and "Peak Results Table in ChromRes Register" in the Help/Commands menu of the ChemStation. Note for users experienced with ChemStation macro development - The ".XLS" workbook is a dump of a combination of ChromRes and each ChromReg register. "Sheet1" is the "header" of ChromRes, while each of the other sheets corresponds to a "Table" from ChromRes or ChromReg. In each table sheet the 1st two columns are the table header items, while the rest of the columns are the body of the table. If you follow "links" you need to account for the data starting on row #2 in the sheet, while it started on row #1 in the internal tables. 14. Barcode Reader use with methods ----------------------------------- Use of the barcode reader is always disabled for single method runs. Workaround: To use the barcode reader for single runs, use a single line sequence. The Sequence Parameters screen allows you to select whether the barcode reader is enabled or disabled for the sequence. 15. The 7673A/B/C family of ALS products are no longer supported ------------------------------------------------------------------------ The 7673A/B/C family of ALS products are no longer supported by OpenLAB ChemStation Edition. 16. 35900E A/D Converter ------------------------- Support Matrix ___________________________________ | Instrument | 35900E | |_____________ |_________________ | | | | | 7890, 7820A | Not Supported | |_______________ |_____________ | | | | | 6890 RC.NET | Not Supported | | 6850 RC.NET | | |______________ |_________________ | | | | | 6890 Classic | Supported | | 6850 Classic | | |_____________ |_________________ | | | | | LC Classic | Supported | | LC RC.NET | Supported | |____________ |_______________ | | | | | CE Classic | Supported | | CE RC.NETc | Not Supported | |__________ |_________________ | 35900 configuration ------------------- When configuring a 35900E, the user can select "ChemStation displays status of ready signal" and "ChemStation waits for ready signal" in the External Start/Stop tab of the 35900E Options. When these are selected, the ChemStation will wait for all external instruments to become ready before allowing the start of the run. Regular restart of the 35900E required -------------------------------------- The highest integer that the internal counter for the power-on timer can store is equivalent to a time of two weeks. If the instrument is online for more than two weeks, the internal counter will cause an overflow. Symptoms are missing data points at the beginning or end of a 35900 signal or graphic artifacts in the online plot. It is recommended to power cycle the instrument once per week. Minimum overall runtime required -------------------------------- When the 35900E is configured in a GC system as 3rd and 4th signal, the overall runtime must be greater than the injection cycle time. When the overall run time is less than the injection cycle time the 35900 will generate a stop on the APG remote start stop bus that will result in a vial being left in the injector. The result is incorrect vials will be injected during sequence operation. 17. Retention Time Locking Notes --------------------------------- - Regarding 'ReLock current method...': RTL software can automatically read and update only 7890 and 68xx methods that are in constant pressure mode. For 7890 and 68xx methods not in constant pressure mode (e.g. constant flow), you must press the 'Calculate' button to view the suggested locking pressure, then enter that pressure in the appropriate 'Instrument' screen. - A sample of a retention timetable is included with the RTL software. It is called example.rtt. You may want to use this as a model for creating one of your own. - Regarding 'Import RT Table...': The format of the file to import is important. RTL software assumes that the first line of the file is the table title, the second line of the file contains the 10 column titles and the remaining lines of the file contains the retention times and other compound information. All items on a line must be separated by the tab character (this allows you to have comma characters in compound names.) Column 1 of the compound information lines must be a number. Column 4 of the compound information lines must have element names and quantities in the special format to enable you to specify elemental and detector information in your searches. To see an example of this format, export example.rtt and view it in a text editor, like notepad.exe. 18. LC/MS ChemStation Instrument Models supportability matrix ----------------------------------------------------------------------------- 1. LC/MS Instrument Models G1946A/B/C/D and G1956A/B are not supported by OpenLAB CDS ChemStation Edition. 2. OpenLAB CDS ChemStation Edition will support LC/MS Instrument Models G6110A, G6120A/B, G6130A/B, G6140A, and G6150B. 3. Minimum required LC/MS firmware is 3.03.40. 19. Power Management and Screen Savers and the ChemStation ----------------------------------------------------------- In general screen savers should not be used together with the ChemStation except simple screensavers such as blank screen may be used. OpenGL screen savers should not be used. Screen savers can cause undue loading of the CPU that may result in the loss of data from the GC during high data collection periods. The Aero theme that is available in Windows 7 can cause graphic artifacts in various ChemStation screens like Online Plot or Data Analysis. To avoid these effects select a Windows Classic Theme for your desktop or disable the Desktop Window Manager Session Manager service. A similar situation has been observed on computers with Windows XP. In this environment setting the graphic hardware acceleration to "off" solves the issue. Advanced power management features of the PC BIOS must be disabled for systems that have to perform instrument control and data acquisition. See the Manual "Configure and Maintain Your ChemStation Computer" for more details. Any Energy Star compliant PC should have the Energy Save features disabled to prevent the loss of data during high data collection periods. Problems may also be exhibited by I/O error with the instruments. I/O errors will result in the loss of data. They can be found in the log book. 20. Support Statement for Microsoft Windows Vista ------------------------------------------------- Starting with OpenLAB CDS Rev A.01.05, Microsoft Windows Vista is no longer supported. _______________________________________________________________________________ 3 Known Issues IMPORTANT Windows hotfixes KB2600907 and KB2577795, or hotfixes that officially supersede these hotfixes from Microsoft must be installed on the machine hosting the Data Store or OLSS server. Install hotfixes: KB Article Number(s): 2600907 Language: All (Global) Platform: x64 Always install hotfix KB2600907 (http://support.microsoft.com/kb/2600907). KB Article Number(s): 2577795 Language: All (Global) Platform: x64 If you plan to use OpenLAB Data Store: Install hotfix KB2577795 (http://support.microsoft.com/kb/2577795) For a full list of known issues in OpenLAB CDS ChemStation Edition see the M83XXAA-SSB.html file located under the support\SSB folder on disk 2. ________________________________________________________________________________ 4 Obtaining Support Thank you for purchasing OpenLAB CDS. If you have purchased a workstation configuration please contact your local sales and support center for support with any aspect of your OpenLAB CDS system. To determine the phone number of your local sales and support team visit http://www.chem.agilent.com/en-US/ContactUS/Pages/ContactUs.aspx. If you have purchased a networked configuration and have purchased a Software Maintenance Agreement (SMA) from Agilent, you are also entitled to priority support at https://www.agilent-labinformatics.com/support. Register your SMA and receive a number of benefits including: - Online Ticket Submission, Prioritization and Status Tracking - Online Feature Request Submission and Tracking - Readily available self-help tools and useful links Again, thank you for your business. We look forward to supporting all of your laboratory needs. NB: Microsoft and MS are registered trademarks of Microsoft Corporation. Windows XP, Windows Vista, and Windows 7 are trademarks of the Microsoft Corporation. Flexera Software, FlexNet and SubscribeNet are trademarks or registered trademarks, respectively of Flexera Software, Inc.